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Substrate solution is then added and color develops in proportion to the amount of Mouse DBN1
The plate has been pre-coated with Mouse MSR1 antibody
Substrate solution is then added and color develops in proportion to the amount of Mouse TNNT2
Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Mouse SELP are added to the micro ELISA plate wells
Then a biotinylated detection antibody specific for CFD and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
Human CH25H (Cholesterol 25-hydroxylase) ELISA Kit OSF2 Substrate solution is then addedThe Human CH25H (Cholesterol 25 hydroxylase) ELISA Kit measures CH25H in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti CH25H antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated
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